procedure for the proposed cholesterol reference method

  • 1.95 MB
  • English
Clinical Chemistry Division, Center for Environmental Health, Centers for Disease Control, Public Health Service, U.S. Dept. of Health and Human Services , Atlanta, Ga
Cholesterol., Cholesterol -- stand
Statementby Irma W. Duncan, Alan Mather, Gerald R. Cooper.
ContributionsMather, Alan., Cooper, Gerald R., Center for Environmental Health (U.S.). Clinical Chemistry Division.
The Physical Object
Pagination1 v. (various pagings) :
ID Numbers
Open LibraryOL17656553M

Get this from a library. The Procedure for the proposed cholesterol reference method. [Irma W Duncan; Alan Mather; Gerald R Cooper; Center for Environmental Health (U.S.). The AK RMP was proposed more than 60 years ago and is considered the secondary reference method for analyzing cholesterol.

It has been used internationally as a reference procedure for measuring cholesterol at CDC, CRMLN laboratories, and in Cited by: 7. For more than 50 years, the Centers for Disease Control and Prevention has standardized cholesterol measurements by use of a spectrophotometric reference measurement procedure (RMP) 3 ().This procedure is a modified version of the Abell–Levy–Brodie–Kendall (AK) colorimetric method and is accepted as the accuracy point to which clinical cholesterol measurements are by:   Isotope-dilution mass spectrometry (ID-MS) is a reference measurement procedure for cholesterol quantification approved by NIST.

Details procedure for the proposed cholesterol reference method PDF

Inthe first GC-ID-MS method was by: 8. 1. Introduction. Ina new method for serum cholesterol measurement using cholesterol dehydrogenase (CD, EC No. not certified), and cholesterol esterase (CE, EC ) with endpoint in the UV (CD-UV method) was reported by Kayamori et CD-UV method is accurate and comparable to the Abell-Levy-Brodie-Kendall (ALBK) method, which has been adopted as a reference method Author: Ikunosuke Sakurabayashi, Tatsuo Watano, Nobuo Inaba, Koji Kishi, Kazuaki Yamashita, Yuzo Kayamori.

A GC–IDMS reference method for the measurement of total cholesterol in human serum was implemented. The reference method was used to assign reference values to frozen serum samples of five French PT schemes. Our results show that cholesterol measurements are positively biased, mean bias being + % ± %.

The most widely used methods did. The cholesterol reference method is a modification of the Abell-Levy-Brodie-Kendall chemical method (18). The cholesterol reference method uses chemical hydrolysis with alcoholic KOH, hexane extraction, and color development with Liebermann-Burchard reagent (acetic anhydride-acetic acid-sulfuric acid).

Download procedure for the proposed cholesterol reference method PDF

The procedure for the proposed cholesterol reference method. Centers for Disease Control Publication, Atlanta.

22 De Stefane F. Devine OJ, Flanders WD, et al. Serum levels of ,Tetrachlorodibenzo-p-dioxin in U.S. Army Vietnam-era veterans. A complete cholesterol test is also called a lipid panel or lipid profile. Your doctor can use it to measure the amount of “good” and “bad” cholesterol and triglycerides, a type of fat, in.

The “gold standard” or reference method for LDL-C is called the “beta quantification” procedure, which requires the use of ultra-centrifugation to first remove chylomicrons and VLDL, followed by measurement of cholesterol in the LDL and HDL containing “bottom” fraction, selective precipitation of LDL, and measurement of the HDL cholesterol (HDL-C) in the supernatant.

Reference System consisting of reference methods and reference materials with target values assigned by the reference methods, Traceability procedure in which participants use the reference materials created in the previous step to calibrate their tests or to verify the analytical accuracy and precision of their testing system.

Proposed serum cholesterol reference measurement procedure by gas chromatography-isotope dilution mass spectrometry. Edwards SH(1), Kimberly MM, Pyatt SD, Stribling SL, Dobbin KD, Myers GL.

Author information: (1)Clinical Chemistry Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and. The calculation was actually proposed for use in epidemiologic studies but was later rapidly adopted and became the method of choice by routine clinical laboratories, in part for economic reasons.

Shortcomings of the procedure were addressed in the original publication. These includes: Overestimation of very low-density lipoprotein cholesterol.

over should have a fasting lipoprotein profile (total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride) once every five years to screen for coronary heart disease risk.2 PRINCIPLES OF PROCEDURE The use of enzymes to assay cholesterol has been studied by many investigators.3,4 This reagent is based on the formulation of Allain.

Proposed Serum Cholesterol Reference Measurement Procedure by Gas Chromatography–Isotope Dilution Mass Spectrometry. Clinical Chemistry57 (4), DOI: /clinchem Weiyan Zhou, Hongxia Li, Jun Dong, Shu Wang, Ruiyue Yang, Chuanbao Zhang, Wenxiang Chen. () Siekmann L Reference methods for total cholesterol and total glycerol.

Eur J Clin Chem Clin Biochem ; () Siekmann L, Huskes KP, Breuer H Determination of cholesterol in serum using mass fragmentography: a reference method in clinical chemistry.

Z Anal Chem ; (). Methods: We evaluated the performance of the novel method within an independent cohort of 23 patients with LDL-C measured by the gold standard β-quantification reference method. human serum was determined by the enzymatic method using the LAMBDA ™ UV/ Vis Spectrophotometer and UV Lab ™ software.

Description procedure for the proposed cholesterol reference method EPUB

Principle. The cholesterol esters of the sample are hydrolyzed by cholesterol esterase. 4-Cholestenone and H. are then formed from the released free cholesterol by cholesterol oxidase. CDC offers customized QA and standardization programs to help laboratories improve the quality and reliability of their measurement procedures.

The specific CDC services that are offered provide: reference materials, proficiency testing, training, guidelines, and consultations. Although the JCTLM, the international authority on pathology laboratory values, lists the CDC Lieberman-based “Abell-Kendall’ method for cholesterol as a reference method for defining the true cholesterol value, definitive methods such as isotope dilution mass spectrometry are ideal for defining the calibration point for assays.

In the presence of oxygen, free cholesterol is oxidized by cholesterol oxidase to cholestenone. The H2O2 reacts in the presence of peroxidase (POD) with phenol and 4-aminophenazone to form an o-quinone-imine dye. The intensity of the color is proportional to the cholesterol concentration and is measured photometrically.

(1.) Abell LL, Levy BB, Brodie BB, Kendall FE. A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity, J Biol Chem ; (2.) Dunkan IW, Mather A, Cooper GR.

The procedure for the proposed cholesterol reference method, Clinical Chemistry Division, Centers for Disease Control. Method A resulted in a value of +/ (SE) mg cholesterol/g of yolk for the National Institute of Standards and Technology (NIST) reference (cholesterol in whole egg powder) as compared with.

Performance of a Designated Comparison Method for HDL-Cholesterol for Use in the Cholesterol Reference Method Laboratory Network. Clin Chem. ; Second Report of the Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel II).

NIH Publication No. ; September This revenue procedure is being released in conjunction with Rev. Proc.I.R.B.which provides procedures and guidance on the process of requesting assistance from the U.S.

competent authority under the provisions of U.S. tax treaties. A proposed version of this revenue procedure was released for public comment. METHODS ANALYSIS Methods analysis is the study of how a job is done.

Whereas job design shows the structure of the job and names the tasks within the structure, methods - Selection from Operations Management: An Integrated Approach, 5th Edition [Book].

used methods for cholesterol determinations in clinical laboratories. For a true cholesterol value between mg/dl and mg/dl, the aca method had an upward bias of between and percent, while the SMAC method 2 The coefficient of variation is the standard devi at ion of aprobabi [ i ty distributions as a per.

The results are displayed in Table 2. The comparison-ofmethods scatter plot and the linear regression analysis are shown in Fig.

1, with the modified HDL-C Reference Method values on the x-axis. All assays correlated highly with the modified reference procedure (r. The accuracy of the LC IDMS method for the determination of total cholesterol in serum was checked with the five different serum-based control materials, for which the target values were based on Definitive Methods in the case of the NIST materials and on GC IDMS Reference Method-based target values supplied by the manufacturer.

Cholesterol content as estimated by the standard method was to and to mg/ g in clarified cow and buffalo ghee, respectively, whereas, the cholesterol content in the.

The expression X TG ( X TG when measurements are reported in millimoles per liter) also produced a more accurate estimate of very low-density lipoprotein cholesterol relative to values obtained by the standard Lipid Research Clinics procedure for this component.of a bacterial cholesterol oxidase, various procedures were sooft developed for the determination of chol-esterol with the aid of this enzyme.

In the meantime, in many laboratories, the enzymatic methods have replaced the older procedures mentioned above. They appear to be more specific and better suited for mechanisation.The second method is based on partial least square (PLS) of all three colors R, G and B.

Accuracy of the method was validated using UV-visible spectrophotometry as a reference method.